Fig. S3.

Lineage tracing of the ap-GAL4 driver. (A) G-TRACE–mediated cell lineage analysis to irreversibly label all cells born in the ap-GAL4 domain. Larval wing primordia were stained for DAPI (gray), Cut (blue), RFP (red), and GFP (green). Note expression of GFP but not RFP in the myoblast cell population. (B and C) Larval wing primordia of the indicated genotypes stained for DAPI (blue), Twist (red), and dMMP1 (green). Note that the myoblast cell population, labeled by the expression of Cut, was not ablated by reaper. (Scale bars, 50 µm.)