Fig. S5.

The B. subtilis SecA–G. thermodenitrificans SecYE cocrystal structure (PDB ID code 5EUL). SecA is shown in light gray, SecYE is in dark gray, and the OmpA peptide substrate inserted at the end of the THF is shown in pink. For clarity, the nanobody crystallized with the complex has been omitted (10). The FRET-mapped regions in the presence of ATP-γS of the beginning portions of the PhoA substrate for residues 2, 22, 37, or 45 are shown in blue, green, yellow, or red, respectively. Mapping was done as described in Fig. S4. Overlap regions of PhoA residues 22, 37, and 45 are shown in olive, and overlap regions of 37 and 45 are shown in orange.