Fig. 5.

Molecular basis for LlPC insensitivity to acetyl-CoA activation. (A) Surface area burial at the BC–PT interface in the structures of LmPC c-di-AMP complex, SaPC free enzyme, SaPC CoA complex, and LlPC c-di-AMP complex. (B) Sequence alignment of residues in the BC–PT interface. Four residues that are unique to LlPC are highlighted in cyan. (C) Detailed interactions at the BC–PT interface, with the four residues that are unique to LlPC labeled in red. (D) The quadruple mutant of LlPC is sensitive to acetyl-CoA and is activated by about twofold. The reactions contained 0.4 μM of the mutant protein and 10 mM pyruvate.