Table S1.
Summary of crystallographic information
| Structure | c-di-AMP complex | c-di-AMP complex (G746A mutant) | Free enzyme |
| Space group | P1 | P1 | P3212 |
| Cell dimensions | |||
| a, b, c, Å | 97.3, 130.4, 133.4 | 97.2, 130.4, 134.3 | 139.7, 139.7, 610.5 |
| α, β, γ, ° | 66.1, 89.1, 70.6 | 66.0, 88.7, 70.1 | 90, 90, 120 |
| Resolution* | 50–2.3 (2.38–2.30) | 50–2.0 (2.07–2.00) | 50–3.1 (3.15–3.10) |
| Rmerge, % | 8.4 (49.8) | 5.9 (49.2) | 9.5 (76.0) |
| I/σI | 8.6 (1.7) | 12.5 (1.7) | 12.0 (1.7) |
| Redundancy | 2.0 (1.9) | 2.3 (2.0) | 4.5 (4.3) |
| Completeness | 90.0 (89.5) | 92.3 (90.4) | 99.7 (99.8) |
| R, % | 18.5 (27.4) | 17.6 (25.9) | 20.2 (29.6) |
| Rfree, % | 22.1 (30.8) | 20.2 (28.2) | 24.8 (34.9) |
| Average B factors | |||
| Protein | 39.8 | 39.5 | 83.9 |
| Ligand/ion | 37.1 | 34.8 | 106.2 |
| Water | 31.4 | 33.4 | — |
| Rms deviation bond lengths, Å | 0.015 | 0.013 | 0.013 |
| Rms deviation bond angles, ° | 1.7 | 1.6 | 1.6 |
| Ramachandran plot | |||
| Favored | 97.6% | 97.5% | 84.2% |
| Outliers | 0.07% | 0.07% | 0.18% |
| PDB ID code | 5VYZ | 5VZ0 | 5VYW |
*
Values in parentheses are for the highest-resolution shell.