. 2017 Aug 14;114(35):E7226–E7235. doi: 10.1073/pnas.1704756114

Table S3.

Plasmids used in this study

Plasmid	Properties	Antibiotic resistance	Source
pRV300	Suicide vector in L. lactis. Replicates in E. coli	Ap^r, Em^r	Ref. 56
pRV300-ΔpycA	pRV300 with fused US fragments and DS fragments of either side of pycA for targeted deletion of pycA	Ap^r, Em^r	This study
pRV300-pycA^Y715T	pRV300 with pycA^Y715T fragment for targeted change of amino acid Y715T in PycA	Ap^r, Em^r	This study
pRV300-pycA^G746A	pRV300 with pycA^G746A fragment for targeted change of amino acid G746A in PycA	Ap^r, Em^r	This study
pGh9	Previously called pPNG904. A pGhost9::ISS1 derivative containing a Cm^r gene inserted into the EcoRI and SalI sites, replacing ISS1. Replicates in L. lactis at 30 °C.	Em^r, Cm^r	Ref. 57
pGh9-pycA	pGh9 with entire pycA gene replacing the Cm^r gene for complementation of ΔpycA and overexpression in OS2	Em^r	This study
pGh9-pycA^Y715T	pGh9 with entire pycA^Y715T gene replacing Cm^r gene for overexpression in OS2	Em^r	This study
pGh9-pycA^G746A	pGh9 with entire pycA^G746A gene replacing Cm^r gene for overexpression in OS2	Em^r	This study

US, upstream; DS, downstream.