Fig. 4.

Three-dimensional reconstructions of asymmetric structures. (A and D) High-resolution structural models of a spiral-shaped and a duckling-shaped structure. (B and E) Class averages produced by cumulating similar projections of the spiral-shaped and duckling structures generated with a 6- and 5-nm labeling spacing, respectively. (C and F) Three-dimensional reconstructions of the spiral and duckling structures. Black and red triangles on colormaps represent the threshold and iso values used for the intensity projections and 3D representation of the structures, respectively.