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. 2017 Aug 15;114(35):E7301–E7310. doi: 10.1073/pnas.1705441114

Fig. 6.

Fig. 6.

KPNB1 positively regulates multiple APC/C family members. (A) Diagram of LC-MS analysis after SILAC. (B) Expression levels of KPNB1 and APC/C-related proteins in the nucleus of SKOV3 and OVCAR3 cells following transfection with KPNB1 siRNA or NC siRNA as assessed by LC-MS. Relative expression ratios in cells treated with KPNB1 siRNAs compared with those treated with NC siRNA are shown. (C) Expression levels of KPNB1 and APC/C-related proteins in 136 human cancer cell lines as assessed by LC-MS and plotted to analyze the correlation between expression levels of KPNB1 and APC/C-related proteins. Pearson’s correlation coefficients for APC8, APC5, APC2, and APC4 and Spearman’s correlation coefficients for APC7 and APC1 are shown. (D) MDA-MB-231 (breast adenocarcinoma), MCF-7 (breast adenocarcinoma), MIA PaCa-2 (pancreas ductal adenocarcinoma), and Huh-7 cells (well-differentiated hepatocellular carcinoma) were transfected with NC siRNA or KPNB1 siRNA. Cell proliferation/survival was then assessed by WST-1 3 d after transfection (n = 4 each, *P < 0.05 vs. all).