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. 2017 Aug 14;114(35):9439–9444. doi: 10.1073/pnas.1707032114

Fig. S6.

Fig. S6.

SPIN does not inhibit murine, bovine, equine, or rabbit MPO. (A and B) Direct binding of SPIN to human recombinant MPO (rMPO) (A) and mouse rMPO (B) determined by SPR. SPIN is able to bind to human rMPO, but not to mouse rMPO. (C) SPIN inhibits human rMPO. “MPO only” shows MPO activity; adding 1 µg/mL SPIN inhibits the activity of MPO similar to azide control. (D) SPIN cannot inhibit mouse rMPO. Adding 50 µg/mL SPIN does not change the activity. Azide is able to completely inhibit the activity of the mouse rMPO. (E–G) SPIN cannot inhibit bovine, equine, and rabbit MPO. Neutrophils from cow, horse, and rabbit were lysed and incubated with 10 µg/mL SPIN (cow and horse) or 9 µg/mL SPIN (rabbit).