Fig. S7.

MPO inhibitor AZM198 is able to cross neutrophil membrane and inhibit intracellular MPO. Polymorphonuclear (PMN) lysate without the inhibitor shows MPO activity. PMNs incubated with 10 µM inhibitor for 1 h at room temperature, washed, and then lysed, are devoid of MPO activity. The activity was comparable to samples which were incubated with the inhibitor after lysis. Thus, the MPO inhibitor AZM198 crosses the neutrophil membrane and is suitable to use for bactericidal assays to test the MPO-dependent killing of bacteria.