Skip to main content
. 2017 Aug 14;114(35):E7348–E7357. doi: 10.1073/pnas.1707304114

Fig. 5.

Fig. 5.

G4 recognizes a variable loop in the S2 connector domain. (A) Structure of G4 Fab bound to a variable loop contained within the S2 subunit. Residues 1171–1187 of MERS S-2P are shown as a ribbon, with the side chain of Asn1176 and two attached N-acetylglucosamine moieties shown as sticks. The variable domains of G4 are shown as a molecular surface. (B) G4 binding interface. Side chains of interacting residues are shown as sticks, with residues substituted in G4-escape variants colored orange. Black dotted line indicates a salt bridge. (C) Sequence alignment of MERS-CoV isolates (green) and other lineage C betacoronaviruses (tan). Bold font indicates N-linked glycosylation sites. (D) Side views of one S2 protomer bound to G4 Fab. On the right, S2 is shown as a molecular surface and colored according to sequence conservation as determined by the ConSurf server using 66 diverse coronavirus sequences (85).