Table S1.

Thermodynamic binding parameters determined by ITC of pAgs for the WT and mutant BTN3A1 B30.2 domain

Group	Stoichiometry (N)	Kd	ΔH, kCal/mol	ΔS, cal/(mol⋅degree)
WT-cHDMAPP	0.97 ± 0.0073	1.0 ± 0.7 μM	−12.1 ± 0.15	−13.3
WT-IPP	1 (fixed value)	4.8 ± 2.4 mM	−5.2 ± 0.4	−1.69
Y352A-cHDMAPP	0.97 ± 0.023	6.2 ± 0.3 μM	−10.2 ± 0.33	−10.5
Y352A-IPP	1 (fixed value)	N.A.	N.A.	N.A.

N.A., not assessed.