Fig. S3.

Tumor growth in BM chimeric mice. (A) Lethally irradiated WT mice received BM transplants from Batf2^−/− or WT littermates. Eight weeks after BM transplant, the chimeric mice were each injected s.c. with 1 × 10⁵ B16-F1 cells, and tumor growth was monitored (Left). Tumor sizes on day 18 are shown (Right). Error bars indicate ±SEM. (B) A flow cytometric analysis was performed on the tumor tissues of BM chimeric mice 2 wk after they received an implantation of B16-F1 cells. The populations of CD45⁺ CD11b⁺ F4/80⁺ macrophages from tumor tissues and their IL-12 p40 expressions were analyzed by flow cytometry. Numbers represent the percentages of F4/80⁺ cells within the CD45⁺ CD11b⁺ cell population (Upper) and of IL-12 p40⁺ cells within the CD45⁺ CD11b⁺ F4/80⁺ cell population (Lower). (C and D) An analysis of T cells within the tumor tissues of BM chimeric mice. Numbers represent the percentage of cells within the tumor-infiltrating CD45⁺ CD3⁺ cell (C) or CD8⁺ T-cell (D) populations. Representative plots from two independent experiments are shown. (E) BM chimeric mice were each injected s.c. with 1 × 10⁶ Lewis lung carcinoma cells, and tumor growth was monitored (Left). Tumor sizes on day 12 are shown (Right). Error bars indicate ± SEM. (F) A photographic image of BM chimeric mice taken 15 d after the mice received an s.c. injection of Lewis lung carcinoma cells (E). Bars show means. In all figure parts, *P < 0.05; **P < 0.01; ***P < 0.001.