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. 2017 Aug 10;114(35):9415–9420. doi: 10.1073/pnas.1709166114

Fig. 2.

Fig. 2.

ARC-seq reveals differences in epimutation frequencies and in the spectrum between yeast RNA polymerase mutants. RNAs from wild-type (WT), E1103G, and ΔRpb9 yeasts were sequenced via ARC-seq, with the number of cDNA copies per RNA molecule required to generate a consensus sequence varied from one through five. (A) Epimutation frequency stabilizes at three cDNAs per RNA molecule, revealing epimutation frequency differences between WT and the two mutants. (B) Comparison of epimutation frequencies observed with one cDNA copy per RNA molecule, corresponding to conventional RNAseq with tag-based error correction, and three cDNA copies per RNA molecule for mRNA (Left) and rRNA (Right). Differences between the mRNA (C) and rRNA (D) mutation spectrums of WT and mutant yeasts are shown. Error bars represent Wilson scores of 95% confidence. *P < 0.01, **P < 10−5, ***P < 10−10, ****P < 10−15.