Fig. 3.
ARC-seq reveals differences in TM after oxidative stress between yeast RNA fidelity mutants and between RNA types. Wild-type yeast was exposed to H2O2, and its RNAs were then sequenced via ARC-seq. (A) Comparison of mRNA mutation frequencies observed with one cDNA copy per RNA molecule, corresponding to conventional RNAseq with tag-based error correction, and with three cDNA copies per RNA molecule. (B) mRNA frequency and spectrum in untreated (ctrl) and 50 μM-treated (H2O2) yeasts. (C) Frequency and spectrum of rRNA in ctrl and 50 μM-treated (H2O2) yeasts. Error bars represent Wilson scores of 95% confidence. *P < 0.05, **P < 10−2, ***P < 10−5, ****P < 10−10, *****P < 10−15.