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Dysfunction of ASI1 and its associated proteins affects the expression of a nonintroinc HC gene. RT-qPCR shows that the ASI1-AIPP1-EDM2 subcomplex is required for the expression of AT4G16870, a TE gene with heavy DNA methylation. A snapshot of DNA methylation in this locus is shown. Relative expression in different mutants was first normalized to ACTIN2 and then to Col-0 plants. Error bars indicate SD; n = 3. **P < 0.01, Student’s t test.
