Fig. S8.

AIPP1 prevents silencing of the 35S-SUC2 transgene locus. (A) The root growth phenotype of the aipp1-1 mutant in the 35S-SUC2 transgene background in half-strength Murashige and Skoog MS medium supplemented with 1% sucrose or 1% glucose. asi1-1 and edm2-6, which were already in the 35S-SUC2 transgene background, were grown as parallel controls. (B) RT-qPCR results showing that expression of the SUC2, HPTII, and NPTII transgenes was significantly reduced in aipp1-1, asi1-1, and edm2-6 mutant plants in comparison with the WT parental 35S-SUC2 plants. All plants were of the 35S-SUC2 background. Relative expression of the transgenes was first normalized to ACTIN2 plants and then to 35S-SUC2 plants. Error bars indicate SD; n = 3. **P < 0.01, Student’s t test.