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. 2017 Aug 14;114(35):E7377–E7384. doi: 10.1073/pnas.1710683114

Table S1.

Primers used in this study

Primer Sequence (5′-3′) Purpose
AIPP1-geF caccTGTTAATCGGTAGAGATGGACATGTTG Gateway cloning of AIP genomic DNA
AIPP-geR CCGATAAGGAAAACCTTTCAGGTCGT
AIPP1-CDS-NdeIF ctgCATATGATGGAAGAATCTGTAGCATCTGAAG ASI1 Y2H constructs
ASI1-CDS-EcoRIR agtGAATTC aggaagtttcagcttgaagttaatc
EDM2-CDS-XmaIF attCCCGGGATGACGTTCGTTGACGATGATGAAG EDM2 Y2H constructs
EDM2-CDS-PstIR gacCTGCAGGTCATTAATCCAACCGCCAGATCGA
EDM2-CDS-XhoIR gagCTCGAGGTCATTAATCCAACCGCCAGATCGA
AIP1-CDS-EcoRIF agtGAATTCATGGCGACTCCGGAAGAAGTAGC AIP1 Y2H constructs
AIP1-CDS-BamHIR acgGGATCCCCGATAAGGAAAACCTTTCAGGTCG
Y3HAarI-EDM2 F aggattattCACCTGCagagAACCATGACGTTCGTTGACGATGATG Y3H constructs
Y3HAarI-EDM2 R aggattattCACCTGCagaggctgTTATCAGTCATTAATCCAACCGCCAG
Y3HAarI-ASI1 F aggattattCACCTGCagagAACCATGGAAGAATCTGTAGCATCTG
Y3HAarI-ASI1 R aggattattCACCTGCagaggctgTTATCAAGGAAGTTTCAGCTTGAAGTTAATCC
Y3HAarI-ADH Terminator F aggattattCACCTGCagagCAGCCAAGCTAATTCCGGGCG
Y3HAarI-ADH Terminator R aggattattCACCTGCagagGAACCCGGTAGAGGTGTGGTCA
Y3HAarI-Met promoter F aggattattCACCTGCagagGTTCGAATCCCTTAGCTCTCA
Y3HAarI-Met promoter R aggattattCACCTGCagagCCATTGCGGCCGCCACCTTTCTC
Y3HAarI-AIPP1 F aggattattCACCTGCagagATGGCGACTCCGGAAGAAGTG
Y3HAarI-AIPP1 R aggattattCACCTGCagagTACCTCACTACCGATAAGGAAAACCTTTCAGG
Y3HAarI-GFP F aggattattCACCTGCagagATGGtagatctgactAGTaaaggagaag
Y3HAarI-GFP R aggattattCACCTGCagagTACCTCACTAtttgtatagttcatccatgcc
Y3HAarI-PGK Terminator F aggattattCACCTGCagagGGTAAGGAATTGCCAGGTG
Y3HAarI-PGK Terminator R aggattattCACCTGCagagCTAGGCTTTAACGAACGCAGAATTTTCG
Y3HAarI-ASI1 alone R aggattattCACCTGCagagCTAGTTATCAAGGAAGTTTCAGCTTGAAGTTAATCC
Y3HAarI-EDM2 alone R aggattattCACCTGCagagCTAGTTACTAGTCATTAATCCAACCGCCAG
AIP1-gwF CACCATGGCGACTCCGGAAGAAGTAGC Split luciferase constructs
AIP1-gwR CCGATAAGGAAAACCTTTCAGGTCG
RPP7-5′qF ATGTGTGATTCTTCCCTAAGAGG RPP7 RT-qPCR
RPP7-5′qR CTTGATCGCGCCAAGGA
RPP7-3′qF GATGGGTTGGAAGAGTGGATAG
RPP7-3′qR TGCAATTGTGGAAACCCATTAG
305410–3′qF GGTCAACGAGCAGATTTGAAAG AT3G05410 RT-qPCR
305410–3′qR CGCAACAAACGGCTACATTAT
305410–5′qF ATTCAACCGACGGCTTCTT
305410–5′qR CCTGGCGATAAACCGAGTATAA
IBM1-3′qF TGAAAAGACCAAATATTGTGAGGAC IBM1 RT-qPCR
IBM1-3′qR caaaagatgatgttaattgaaaactactac
IBM1-5′qF AGGGAATTAGGATTGGGAAAAGG
IBM1-5′qR CGCTCTTTTGACATTGATGGC