Fig. 3.

Deletion of the TAT secretion system does not eliminate VgrG3 toxicity in E. coli. VgrG3 truncation including the cryptic periplasmic localization signal was expressed in knockouts of each component of the TAT system. VgrG3 (632-END) was expressed from an arabinose-inducible promoter, where expression is tightly repressed in the presence of glucose (black bars) and strongly expressed in the presence of arabinose (gray bars). Data shown represent the average of at least three experiments. Error bars represent 1 SD.