Table 1. Description of the strains and plasmids used in the study.
| Name, relevant characteristics | Reference | |
|---|---|---|
| E. coli strains | ||
| DH5α | LuxS supE44 ΔlacU169, (φ80 lacZΔM15) hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Invitrogen |
| DH5α-H | LuxS supE44 ΔlacU169, (φ80 lacZΔM15) hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Hallatschek et al. (2007) |
| MS613 | MG1655 K-12 reference (F-lambda- ilvG- rfb-50 rph-1) strain | Bachmann (1996) |
| MS427 | MG1655 Δflu | Reisner et al. (2003) |
| MS428 | MG1655 Δfim | Kjærgaard et al. (2000) |
| MS528 | MG1655 Δflu,Δfim | Kjærgaard et al. (2000) |
| RMV340 | MG1655cps::tet | Beloin et al. (2006) |
| RMV359 | MG1655fliC ::kan | This study |
| RMV612 | MG1655csgAB::kan | This study |
| S. pombe strains | ||
| PG3732 | h+ ura4D::mCherry | G Thon |
| PG3733 | h+ ura4D::YFP | G Thon |
| B. subtilis strains | ||
| 168phymKATE | eps−: epsG | van Gestel et al. (2014) |
| 168phymGFP | eps−: epsG | van Gestel et al. (2014) |
| Plasmids | ||
| pVS130 | eCFP | V Sourjik |
| pVS132 | YFP | V Sourjik |
| pKD4 | ApKm, kan cassette template | Datsenko and Wanner (2000) |
| pKD46 | Ap, λ Red recombinase | Datsenko and Wanner (2000) |
| pCP20 | ApCm, FLP recombinase expression | Datsenko and Wanner (2000) |
| pACYC184 | Cloning vector, CamR | Chang and Cohen (1978) |
| pHHA13 | Wild-type fim gene cluster in pACYC184 | H Hasman |
Abbreviations: CFP, cyan fluorescent protein; YFP, yellow fluorescent protein.