Table 1. Primers and probes used in tick species determination and pathogen screening.
| Primer/probe name | Target name | Nucleotide sequence (5′→3′) | Reference |
|---|---|---|---|
| Real-time PCR | |||
| Bbsl-ospA-F | B. burgdorferi ospA | AATATTTATTGGGAATAGGTCTAA | 7 |
| Bbsl-ospA-R | CACCAGGCAGCAAATCTACTGA | ||
| Bbsl-ospA-P | [6FAM]-TTAATAGCATGTAAGCAAAATGTTAGCA-[DDQ1] | ||
| Bm-fla-F | B. miyamotoi flagellin | AGAAGGTGCTCAAGCAG | 8 |
| Bm-fla-R | TCGATCTTTGAAAGTGACATAT | ||
| Bm-fla-P | [6FAM]-AGCACAACAGGAGGGAGTTCAAGC-[DDQ1] | ||
| IXO-I2-F4 | Ixodes spp. ITS2 | TCTCGTGGCGTTGATTTGC | 9 |
| IXO-I2-R4 | Ixodes spp. ITS3 | CTGACGGAAGGCTACGACG | |
| Ipe-I2-P4 | I. persulcatus ITS4 | [FAM]-TGCGTGGAAAGAAAACGAG-[BHQ1] | |
| Iri-I2-P4 | I. ricinus ITS5 | [VIC]-TGCTCGAAGGAGAGAACGA-[BHQ1] | |
| Real-time RT-PCR | |||
| F-TBEV1 | 3′-non-coding region of the TBEV genome | GGGCGGTTCTTGTTCTCC | 10 |
| R-TBEV1 | ACACATCACCTCCTTGTCAGACT | ||
| P-TBEV-WT | [FAM]-TGAGCCACCATCACCCAGACACA-[TAMRA] | ||
Abbreviations: internal transcribed spacer 2, ITS2; outer surface protein A, ospA; reverse transcription-PCR, RT-PCR; tick-borne encephalitis virus, TBEV.