Figure 4.

A multiplex PCR assay for simultaneous detection of the SEA deletion α⁰-thalassemia and a T allele of the single-nucleotide polymorphism (SNP) rs3760053 using combined gap-PCR (for SEA deletion) and allele specific PCR (for T allele). The locations and orientations of primers used were depicted. Gel electrophoresis represented results of the multiplex PCR amplification. ‘M’ is the λ/HindIII size markers, whereas 1=homozygote α⁰-thalassemia (Hb Bart’s hydrops fetalis); 2, 3, 4=non α⁰-thalassemia carriers carrying T alleles with S, M, L polymorphisms, respectively; 5 and 6, and 7 and 8=α⁰-thalassemia carriers with T allele of L and M polymorphisms, respectively.