. 2017 Apr 6;62(8):747–754. doi: 10.1038/jhg.2017.41

Table 3. Validation of a multiplex HRM analysis for simultaneous identification of the SEA deletion α⁰-thalassemia and genotyping of the SNP rs3760053 in 211 individuals as compared to the conventional gap-PCR assay.

Multiplex HRM analysis
SEA deletion α⁰-thalassemia	SNP rs3760053 genotyping	No	Gap-PCR assay for SEA deletion α⁰-thalassemia	No
Negative	T/T	126	αα/αα	134
Negative	T/G	8
Positive	T/G	66	αα/—^SEA	71
Positive	G/G	5
Positive	G/G	6	—^SEA/—^SEA	6
Total		211	Total	211

Abbreviations: HRM, high-resolution melting; SNP, single-nucleotide polymorphism.

Table 3. Validation of a multiplex HRM analysis for simultaneous identification of the SEA deletion α0-thalassemia and genotyping of the SNP rs3760053 in 211 individuals as compared to the conventional gap-PCR assay.