Table 2.
Archaeal 16S rRNA gene clone libraries
| Phylogenetic group | In silico T-RF (No. of clones)a | Clone type (GenBank Accession Number) | Closest species | Similarity (%) | ||
|---|---|---|---|---|---|---|
| CK | 0.5 mM | 25 mM | ||||
| Methanosaetaceae | 228 (2) | A4-6 (KJ735834) | Methanosaeta harundinacea 6Ac; NR_102896 | 99 | ||
| 495 (12) | 495 (11) | 495 (10) | A18-26 (KJ735844) | 96 | ||
| 791 (1) | A80-6 (KR297252) | 82 | ||||
| Methanomicrobiaceae | 186 (3) | 186 (12), 187 (1) | A4-7 (KJ735835) | Methanoculleus receptaculi ZC-2; NR_043961 | 99 | |
| 186 (2) | A18-28 (KJ735845) | 95 | ||||
| Methanoregulaceae | 84 (9) | A4-21 (KJ735839) | Methanolinea tarda NOBI-1; NR_028163 | 98 | ||
| 84 (1), 393 (1) | A80-2 (KJ735846) | 97 | ||||
| Methanobacteriaceae | 93 (1), 95 (6) | A80-8 (KJ735840) | Methanothermobacter thermautotrophicus CaT2; AP011952 | 83 | ||
| 91 (1), 92 (5) | A4-27 (KJ735841) | Methanobacterium beijingense 4-1; AY552778 | 99 | |||
aThe 16S rRNA gene clone libraries were retrieved from the cultures after 421 days of incubation. CK, the culture without hexadecane and sulfate addition; 0.5 mM: the culture only amended with hexadecane and 0.5 mM sulfate; 25 mM: the culture amended with hexadecane and 25 mM sulfate