Figure 6.

ACOT7 depletion sensitizes breast cancer cells to irradiation and anti-cancer drug. (a) Kaplan–Meier curves of overall survival times of patients with breast cancer. Data were obtained from http://kmplot.com/analysis/. Statistical significance was determined using the log-rank test. (b) Box plots comparing ACOT7 expression (as log2 median-centered ratios) in normal breast and carcinoma breast tissues. Dots indicate extreme data values. Data were obtained from http://oncomine.org/. (c and d) MCF7 cells were transfected with Con Si or 10 nM ACOT Si prior to 2 Gy of IR exposure. Relative cell numbers were determined on the indicated days (c). Colony-forming assay was performed after 7 days (d). (e) Immunoblot analysis were performed on MCF7 cells transfected with ACOT7 Si indicated concentrations and exposed to 2 Gy of IR. Actin was used as a loading control. (f and g) MCF7 cells were transfected with Con Si or 10 nM ACOT Si prior to treatment with 10 ng/ml of Doxo. Relative cell number was determined on the indicated days (f). Colony-forming assay was performed after 7 days (g). (h) Immunoblot analysis was performed in MCF7 cells transfected with ACOT7 Si and then treated with 10 ng/ml of Doxo for 2 days. Actin was used as a loading control. SE and LE indicates short and long exposures, respectively. The value represents the mean±S.D. from three independent experiments. *** and ** indicate statistical significance of P<0.001 and P<0.01 by Student’s t-test, respectively