Fig. 6.

CCN2 binding with LRP6 occurs in a HSPGs-dependent Manner. a Dose-dependent promotion of adhesion of HCC cells was determined by incubation of MHCC97H cells in 96-well plates that had been pre-coated with 0–3 μg/ml recombinant human CCN2. b MHCC97H–LRP6-Sh2 exhibited decreased cell adhesion to CCN2 compared to untreated MHCC97H cells, and destruction of HSPGs with heparinase or inhibition of HSPG sulfation with NaClO₃ exhibited almost complete inhibition effect for MHCC97H adhesion to CCN2. c Adhesion of HCC cells was determined following co-incubation of CCN2 with heparin. d LMWH inhibited the interaction between CCN2 and LRP6