Figure 4.
c-Myc promotes human colon cancer cell growth through GCN5. (A) HCT116 cells with c-Myc knockdown (KD) were transfected with or without GCN5. Their proliferation was measured by MTT assay. Error bars represent data from three independent experiments (mean ± SD). (B) Cells in (A) were collected at day 3. The apoptotic cells were analyzed by their expression of annexin V. Percentages of annexin V-positive cells are the average ± SD from three independent experiments. Student’s t-test was used for the statistical analysis. **p < 0.01.