Figure 5.

GCN5 is a target of the transcription factor E2F1 to suppress E2F1-induced cell death. (A, B) HCT116 cells were transfected with E2F1 expression plasmids in the presence of GCN5 knockdown siRNA. The protein expression levels of E2F1 (top), GCN5 (middle), and tubulin control (bottom) were analyzed by Western blotting (A). The cell apoptosis was analyzed 5 days after transfection, and the average percentages of annexin V-positive cells from three independent experiments are indicated (B). (C, D) Dataset from TCGA colon cancer (C) and a microarray dataset (accession No. GSE9348) (D) were used. The levels of E2F1 in normal and colon cancer tissues were compared by two-tailed Student’s t-test.