Fig. 3.

GC cause the translocation of E-cadherin from the apical junction to the cytoplasmic vesicles in polarized T84 cells. Polarized T84 cells were incubated with media only, live GC (10:1) or gentamicin killed GC (100:1) in the apical compartment for 6 h. Cells were fixed and stained for E-cadherin and GC and analysed using confocal microscopy. The cell–cell junction and the cytoplasmic regions were manually selected from confocal images sliced through the apical junction, and the fluorescence intensity of E-cadherin staining in each region was measured using the NIH ImageJ software. The fluorescence intensity ratio (FIR) at the apical junction to that in the cytoplasm was calculated. Shown are representative images (A) and the average FIR (± SD) (B) from three independent experiments (~ 50 cells per experiment). Scale bar, 5 μm. ***P ≤ 0.001. *P ≤ 0.05.