Fig. 4.

GC inoculation increases the lateral mobility between the apical and basolateral membrane, but not the permeability of the apical junction in polarized epithelial cells

A and B. Polarized HEC-1-B were incubated with medium alone (−) or GC (+) for 4 h. Cells were then apically exposed to the CellMask membrane dye, and live time-lapse images were acquired using a confocal microscope. The apical to basolateral fluorescence intensity ratio (FIR) of the CellMask dye over time was determined (B).

C. Epithelial cells were incubated with or without GC for 6 h in the presence of Lucifer yellow or fluorescein dye. The FIR of Lucifer yellow or fluorescein in the apical to that in the basal compartments was determined using a luminometer.

Shown are the representitive images and the average values (± SD) from three independent experiments (~ 10 cells per experiment for B). Scale bar, 5 μm.