Fig. 5.

GC inoculation induces the phosphorylation and redistribution of β-catenin.

A and B. Polarized HEC-1-B cells were incubated apically with or without GC for 4 h. Cells were lysed and subjected to immunoprecipitation using β-catenin-specific antibodies. Immunoprecipitates were analysed by SDS-PAGE and Western blot probing for phosphotyrosine. The blot was quantified by densitometry to determine fold increase over no GC control.

C–H. Polarized HEC-1-B (C–E) and T84 (F–H) cells were incubated with or without GC apically for 6 h. Cells were stained for β-catenin and GC and analysed using confocal microscopy. Fluorescence intensity profiles along a line crossing cells (D and G) were generated to determine the β-catenin FIR at the membrane compared to the cytoplasm (E and H). Shown are representative blots, images, fluorescent intensity of the representative images, and the average FIR (± SD) from three independent experiments. Scale bar, 5 μm. ***P ≤ 0.001; ** P ≤ 0.01.