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. Author manuscript; available in PMC: 2017 Sep 5.
Published in final edited form as: Cell Microbiol. 2013 Jan 21;15(6):1042–1057. doi: 10.1111/cmi.12099

Fig. 9.

Fig. 9

GC transmigration is EGFR dependent. Polarized HEC-1-B cells were untreated or pre-treated with the EGFR kinase inhibitor AG1478 (10 μM) or EGF (10 nM) for 2 and 1 h respectively, and then apically incubated with GC for 6 h in the presence or absence of AG1478 or EGF. The basal medium was collected and plated to determine transmigrated cfu (A). Cells were washed and incubated with gentamicin and lysed, and the bacteria collected and plated to determine invaded GC (B). Shown are the average values (± SD) of more than three independent experiments. *P ≤ 0.05.