Figure 3.

Quantitation of urinary plasminogen plus plasmin. Aliquots of urine from 9 patients were subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis and immunoblotting with anti-plasminogen/plasmin antibodies (n = 3–6). Urine volumes analyzed were optimized for detection of plasminogen and plasmin by immunoblotting (10–300 μl) and are listed at the bottom of the figure. Varying amounts of pure plasminogen (4–43 ng) were included on the same blot to create a standard curve and establish levels of plasminogen and plasmin for each patient. A representative immunoblot (a) and corresponding standard curve (b) are shown for 7 patients. (Two patients consistently lacked a signal.) Line between samples 5 and 6 indicates where the blot was cut. Arrowheads indicate plasminogen (PG) and plasmin (P). Data from 3 to 6 analyses were normalized to urine creatinine (Cr), and values are presented in table format (c) and as a bar graph (d). (e) Correlation between urinary plasminogen plus plasmin per gram of creatinine and urinary albumin (Alb)/Cr. std, standard.