SGI-110 at varying doses (6.1, 12.2, 24.4 mg/kg) or DAC (5mg/kg) was administered once weekly for three weeks (days 1, 8, 15); on day 20 after treatment, tumors were harvested. A)
LINE-1 and NY-ESO-1 promoter methylation by bisulfite pyrosequencing; MAGE-A3/6 by MSP (Representative of two experiments). B)
NY-ESO-1 and MAGE-A3/6 mRNA by qRT-PCR. C) NY-ESO-1 and MAGE-A protein expression by western blotting. Data represent 3 animals/group. *p<0.05 vs. vehicle; **p<0.05 vs. DAC.