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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Cancer Prev Res (Phila). 2017 Jul 28;10(9):514–524. doi: 10.1158/1940-6207.CAPR-16-0335

Figure 3.

Figure 3

a: Micropore selection reveals cells having the greatest protrusion intensity. Protrusion intensity, defined as the square of the perimeter divided by area (P2/A) of each cell, was determined using ImageJ. At least 45 cells were analyzed per group; each dot represents the protrusion intensity observed for a single cells. Mean ± SEM. **, P < 0.01; *** P <0.001.

b: Selection does not bias nuclear cross sectional area as determined by post-migration size analysis. Nuclear area was determined by Lamin B1 immunofluorescence of at least 60 cells per group. Mean ± SEM. ***, P < 0.001.

c: Deformability Cytometry (DC) reveals cell line-specific deformability phenotypes coinciding with highly migratory behavior. Graphs show deformability and diameter of at least 1000 unselected and HM cells. Mean ± SEM. *, P < 0.001.