Figure 2. Glucose uptake in CD4 T cells increases at day 15 post infection.

(A–E) C57BL/6 animals were either infected with 1 × 10⁴ PFU of HSV-RE or left uninfected. (A, B) At day 15 pi animals showing SK lesions and naïve un-infected animals were administered with 2-NBDG (i.v) and glucose uptake by CD4 T cells was measured in DLNs. (A) Representative FACS plots and histogram (MFI) showing the 2-NBDG uptake by CD4 T cell from day 15 pi or naïve animals. For fluorescence minus one (FMO), mice were not injected with 2-NBDG. Cells were gated on live CD4+ T cells (B) Histogram number of CD4 T cells in DLNs with 2-NBDG uptake. (C) DLN from naïve and day 15 pi were stained with CD4 and Glut1 or Isotype. Representative FACS plots with Isotype control and Histogram (MFI) showing GLUT-1 expression by CD4 T cells. Cells were gated on live CD4+ T cells (D, E) Total CD4 T cells were purified from naïve and day 15 pi mice showing SK lesions and equal number of cells were used of extracellular flux analysis (D) Line graph showing changes in Extracellular acidification rates (ECAR) by CD4 T cells following addition of glucose, oligomycin and 2DG and Histograms showing basal glycolysis, glycolytic capacity (E) Histogram showing the ratio of OCR to ECAR. Data represents the mean ± SEM of more than 3 independent experiments for A–C (n = 3–5 mice/group) and 2 independent experiments for D & E (n=5 replicates/group). All the data were analyzed with student’s t test. P≤ 0.0001 (****), P≤0.01(**), P≤0.05(*)