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. Author manuscript; available in PMC: 2017 Sep 5.
Published in final edited form as: Cell Rep. 2017 Aug 22;20(8):1881–1892. doi: 10.1016/j.celrep.2017.08.001

Figure 1. Distribution and neurochemical phenotype of VTA NtsR1 and NtsR2 neurons.

Figure 1

VTA NtsR1 and NtsR2 neurons were visualized by crossing NtsR1Cre and NtsR2Cre mice to a Cre-inducible GFP reporter line. See Fig. S1 and S2A for more detail. A) Rostrocaudal distribution of VTA NtsR1 and NtsR2 neurons with bregma positions per (Paxinos and Franklin, 2001). White arrows denote neurons, yellow arrows denote glial cells. B) Colocalization of glial cell marker S100 (purple) in the VTA with NtsR2 cells (green) and C) NtsR1 cells (green). D) Colocalization of tyrosine hydroxylase (TH, purple) in the VTA with NtsR2 cells (green) and E) NtsR1 cells (green). F) Percentage of NtsR1 and NtsR2 neurons that colocalize with TH. G) Percentage of TH positive neurons that colocalize with NtsR1 or NtsR2. H) Total number of GFP-identified NtsR1 and NtsR2 neurons in the VTA. NtsR1Cre;GFP n=4, NtsR2Cre;GFP n=6. Data represent mean ± SEM, *p < 0.05, ***p < 0.001 determined by unpaired t-test. Scale bars = 100 μm. Abbreviations: ml = medial lemniscus; ip = interpeduncular nucleus; fr = fasciculus retroflexus; MT = medial terminal nucleus of the accessory optic tract.