Figure 4.

Gastric corpus expression of γ-H2AX following tamoxifen administration in WT, Nfkb1^−/−, Nfkb2^−/− and c-Rel^−/− mice. (a) Representative photomicrographs of gastric corpus of WT, Nfkb1^−/−, Nfkb2^−/− and c-Rel^−/− mice treated with 150 mg/kg tamoxifen. Sections immunostained for expression of γ-H2AX. Scale bars 100 μm. (b) Percentage of gastric corpus gland cells expressing γ-H2AX in mice treated with vehicle or 150 mg/kg tamoxifen. Statistically significant differences tested by two-way ANOVA and Dunnett’s test. **P<0.01, ***P<0.001. (b–e) γ-H2AX-positive cells plotted by cell position in the gastric corpus hemiglands of WT (c), Nfkb1^−/− (d), Nfkb2^−/−(e) and c-Rel^−/−(f) mice. Solid line represents distribution of cells in vehicle-treated mice, broken line tamoxifen-treated mice. Shaded area marks region of gland where distribution of γ-H2AX cells differs significantly in tamoxifen-treated versus vehicle-treated mice, P<0.05 by modified median test. N=5 for all groups