Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Bioorg Med Chem Lett. 2016 Dec 2;27(3):620–625. doi: 10.1016/j.bmcl.2016.12.002

Figure 1.

Figure 1

Comparison of pain induction by ω-hydroxy PUFAs, 20-HETE, 20-HEPE, and 22-HDoHE, in naïve rats. (A) Intraplantar injection of the corresponding methyl esters (1 µg in 1:9 EtOH/saline) revealed ω-6 hydroxy PUFA (20-HETE), but not ω-3 hydroxy PUFAs (20-HEPE and 22-HDoHE), changed mechanical withdrawal sensitivity in the von Frey assay (Two Way Analysis of Variance, Holm-Sidak post hoc p < 0.001, n = 6, male Sprague Dawley rats). (B) Intraplantar injection of 20-HETE (1 µg of 20-HETE methyl ester in 1:9 EtOH/saline) decreased mechanical withdrawal thresholds in a dose-dependent manner indicating that pain behavior was localized to the injected (ipsilateral) hind paw (Two Way Analysis of Variance, Holm-Sidak post hoc p = 0.005, n = 6, male Sprague Dawley rats).