Calcium influx assay. (A) Capsaicin, 20-HETE, 20-HEPE and 22-HDoHE-induced Ca2+ influx in mTRPV1-transfected HEK293 cells as a function of time. (B) Quantification of the Ca2+ responses (area under curve, AUC) induced by capsaicin, 20-HETE, 20-HEPE and 22-HDoHE. The experiments were repeated twice each in triplicates with similar results.