Table III.
DNA-microarray analysis of gene expression in resistant A4 cells as compared to the parental Jurkat cell linea
| Reference No. | Signal from A4 Cells | Signal Ratios A4 vs Jurkat (Binary Log Ratio, Folds in Parentheses) | Change p Value | Description of the Gene |
|---|---|---|---|---|
| Top up-regulated genes | ||||
| 203939_at | 252 257 |
8.5 (362) 3.5 (11) |
0.00002 0.021224 |
5′-nucleotidase (CD73) (NT5) |
| 218559_s_at | 217.5 | 6.1 (68) | 0.000189 | Kreisler maf-related leucine zipper homolog (KRML) |
| Signal lower threshold | ||||
| 201218_at | 201.5 248.9 |
5.9 (59.7) 7.1 (137) |
0.00002 0.000023 |
C-terminal binding protein 2 |
| 214254_at | 275.8 53.2 |
4 (16) 3.9 (14.9) |
0.00002 0.000035 |
Melanoma Ag, family A |
| 204379_s_at | 212.2 75 |
3.7 (12.9) 3.7 (12.9) |
0.000078 0.004925 |
Fibroblast growth factor receptor 3 |
| 205691_at | 144.4 93.9 |
2.9 (7.46) 1.7 (3.25) |
0.000618 0.030967 |
Synaptogyrin 3 |
| 203471_s_at | 398.9 64.9 |
2.8 (6.96) 0 (1) |
0.00002 0.330589 |
Pleckstrin |
| 210554_s_at | 346.8 556.1 |
2.8 (6.96) 4.4(21.1) |
0.00003 0.000023 |
C-terminal binding protein 2, clone MGC:1563 |
| 205768_s_at | 201.7 154.2 |
2.7 (6.49) 2.3 (4.92) |
0.00002 0.000241 |
Fatty-acid-coenzyme A ligase, very long-chain 1 (FACVL1) |
| 201220_x_at | 389.7 815.5 |
2.6 (6.06) 3.5 (11.3) |
0.00002 0.00002 |
C-terminal binding protein 2 (CTBP2), transcript variant 1 |
| 201215_at | 134.5 59.5 |
2.6 (6.06) 1.3 (2.46) |
0.00002 0.000068 |
Plastin 3 (T isoform) |
| 218718_at | 1263.8 1212.2 |
2.5 (5.66) 2.4 (5.28) |
0.00002 0.00002 |
Platelet-derived growth factor C |
| 209589_s_at | 142.5 66.7 |
2.5 (5.66) 2.9 (7.46) |
0.00002 0.00225 |
Protein-tyrosine kinase EPHB2v |
| 200704_at | 109.4 99.9 |
2.4 (5.28) 1.8 (3.48) |
0.000023 0.00002 |
Integral membrane protein of lysosomelate endosome, LPS-induced TNF-α factor |
| 205488_at | 1018 184.5 |
2.4 (5.28) 1.2(2.297) |
0.00002 0.000052 |
Granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) |
| 210835_s_at | 361.9 485.2 |
2.4 (5.28) 2.8 (6.96) |
0.000023 0.00002 |
Ribeye/C-terminal-binding protein 2 |
| Top down-regulated genes | ||||
| 205347_s_at | 28.5 23.5 |
−4.9 (0.0334) −2.1 (0.2332) |
0.999759 0.996645 |
Thymosin, β, identified in neuroblastoma cells (TMSNB) |
| 202149_at | 2 10.2 |
−4.6 (0.0412) −2.8 (0.1436) |
0.99998 0.999948 |
Consensus includes 3 part of the gene For enhancer of filamentation (HEF1) |
| 209209_s_at | 4.2 2.3 |
−4.5 (0.0442) −5.3 (0.0254) |
0.999973 0.999448 |
Mitogen-inducible 2 |
| 201670_s_at | 4 19 |
−4.1 (0.0583) −1.7 (0.3077) |
0.99987 0.995927 |
Myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L) |
| 201170_s_at | 6.5 17.4 |
−3.6 (0.0825) −2.3 (0.203) |
0.999853 0.977068 |
Basic helix-loop-helix domain, class B, 2 (BHLHB2) |
| 209210_s_at | 29.7 32 |
−2.9 (0.1339) −3 (0.125) |
0.99998 0.99998 |
Mitogen-inducible gene mig-2, complete CDS |
| 210432_s_at | 34.8 120.5 |
−2.8 (0.1436) −0.7 (0.6155) |
0.99998 0.995519 |
Voltage-gated sodium channel α subunit splice variant SCN3A-s |
| 214464_at | 27.8 48.5 |
−2.6 (0.1649) −0.8 (0.574) |
0.99998 0.99996 |
Ser-Thr protein kinase related to the myotonic dystrophy protein kinase (PK428) |
| 215784_at | 265 130.5 |
−2.5 (0.1767) −2.8 (0.1436) |
0.99998 0.99998 |
CD1E Ag, e polypeptide |
| Death receptors | ||||
| 209295_at | 242.7 185.6 |
0.2 (1.148) 0.4 (1.319) |
0.026698 0.213188 |
TRAIL receptor 2 |
| 202535_at | 283.1 463.6 |
0.1 (1.07171) −0.3 (0.812) |
0.5 0.952736 |
Fas (TNFRSF6)-associated via death domain (FADD) |
| 210654_at | 10.8 11.7 |
0.2 (1.148) 1.1 (2.143) |
0.467656 0.318909 |
TRAIL-R4-B (TRAIL-R4) mRNA |
| 203508_at | 22.3 28.1 |
2.3 (4.924) 2.4 (5.278) |
0.099852 0.354442 |
TNFR2 (75 kDa) TNFR superfamily, member 1B |
| Caspases | ||||
| 209790_s_at | 152.9 204.3 |
−0.7 (0.6155) 0.6 (1.515) |
0.99994 0.354442 |
Caspase 6, apoptosis-related cysteine protease |
| 202763_at | 148 197.5 |
−0.4 (0.757) −0.1 (0.933) |
0.998514 0.725952 |
Caspase 3, apoptosis-related cysteine protease (CASP3) |
| 203984_s_at | 152 276.5 |
−0.1 (0.933) 0.3 (1.231) |
0.5 0.083826 |
Protease Mch6, caspase 9, apoptosis-related cysteine protease |
| 207181_s_at | 166.7 105.8 |
0 (1) 0 (1) |
0.5 0.5 |
Caspase 7, apoptosis-related cysteine protease (CASP7) |
| 207686_s_at | 81.9 45 |
0 (1) −1.2(0.435) |
0.681091 0.558077 |
Caspase 8, apoptosis-related cysteine protease (CASP8) |
| Apoptosis-related genes | ||||
| 201746_at | 84.1 104.2 |
0.5 (1.414) 0.7 (1.6245) |
0.161038 0.050553 |
Tumor protein p53 |
| 205385_at | 29.9 24.5 |
1.3 (2.462) −0.4 (0.7578) |
0.005409 0.330589 |
Human p53-associated mRNA, complete CD |
| 207004_at | 27.6 9.6 |
0.8 (1.741) −2.7 (0.1538) |
0.041201 0.284967 |
Homo sapiens B cell CLL lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant β |
| 215037_s_at | 102.9 68.5 |
0.1 (1.07177) −0.9 (0.5358) |
0.5 0.905721 |
Human Bcl-x β (bcl-x) gene |
| 219350_s_at | 556.6 713.4 |
0.1 (1.07177) −0.1 (0.933) |
0.5 0.60871 |
Second mitochondria-derived activator of caspase (SMAC) |
| 210563_x_at | 148.2 180.2 |
0.3 (1.231) 0.6 (1.515) |
0.161038 0.001077 |
FLICE-like inhibitory protein short, CASP8 and FADD-like apoptosis regulator |
| 208478_s_at | 32 63.7 |
0.1 (1.07177) 0.4 (1.319) |
0.5 0.330589 |
BCL2-associated X protein (BAX) |
| 204493_at | 297.2 220.9 |
0.3 (1.231) 0.2 (1.1486) |
0.111714 0.274048 |
BH3-interacting domain death agonist (BID) |
| Heat shock proteins | ||||
| 117_at | 28.9 7.4 |
0 (1) −2.8 (0.1435) |
0.5 0.603256 |
Human heat-shock protein HSP70B gene |
| 211015_s_at | 487 324.8 |
−0.1 (0.933) −0.2 (0.8705) |
0.5 0.961585 |
Human heat shock protein 70 (hsp70) mRNA |
| 200064_at | 3516.6 3904.4 |
0 (1) 0.6 (1.1557) |
0.5 0.000023 |
Chaperone protein HSP90 β (HSP90β) mRNA |
| Kinases and phosphatases | ||||
| 212046_x_at | 136 150 |
0.4 (1.3195) −0.1 (0.933) |
0.570859 0.583567 |
ERK1 mRNA for protein serine threonine kinase |
| 214693_x_at | 301 380.7 |
0 (1) 0.2 (1.1486) |
0.5 0.124552) |
Phosphatase type 2A |
| 211500_at | 9.2 7.3 |
0.6 (1.1557) 0.3 (1.231) |
0.005409 0.000966 |
p38β MAPK mRNA, complete CD |
| 208351_s_at | 138.1 111.8 |
0.4 (1.3195) −0.5 (0.7071) |
0.004481 0.964215 |
Mitogen-activated protein kinase 1 (MAPK1) |
| 201461_s_at | 77 57.6 |
0 (1) −0.1 (0.933) |
0.545234 0.5 |
Mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2) |
| 210570_x_at | 78.7 207.6 |
0 (1) −0.4 (0.7578) |
0.5 0.861614 |
JNK2β l protein kinase (JNK2B1) |
| 206369_s_at | 7.2 9.4 |
−2 (0.25) −0.2 (0.8705) |
0.992212 0.715033 |
Phosphoinositide-3-kinase γ catalytic subunit (PI3KCG) |
| Adenosine receptors and purine-metabolizing enzymes | ||||
| 205481_at | 50.7 82.8 |
0 (1) 1 (2) |
0.493524 0.558077 |
Adenosine A1 receptor (ADORA1) |
| 205013_s_at | 47.7 101.1 |
0.3 (1.231) 0.1 (1.072) |
0.022932 0.037598 |
Adenosine A2a receptor (ADORA2A) |
| 205891_at | 91.1 215.3 |
−0.2 (0.8705) 0.8 (1.741) |
0.930187 0.023926 |
Adenosine A2b receptor (ADORA2B) |
| 206171_at | 50.2 49.7 |
0.1 (1.072) 0 (1) |
0.5 0.246094 |
Adenosine A3 receptor (ADORA3) |
| 207691_x_at | 34.1 16.7 |
0.5 (1.414) −0.4 (0.7578.) |
0.5 0.219482 |
Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39) |
| 202588_at | 27.7 22.9 |
0.9 (1.866) 0.8 (1.741) |
0.242585 0.753906 |
Adenylate kinase 1 (AK1) |
| 205851_at | 84.2 122.9 |
−0.2 (0.8705) 0.1 (1.072) |
0.838962 0.01416 |
Nucleoside diphosphate kinase |
| 204639_at | 4056.9 3819.5 |
0.2 (1.1486) 0.5 (1.414) |
0.232549 0.000244 |
Adenosine deaminase (ADA) |
| 201695_s_at | 684.9 422.9 |
0.5 (1.414) −0.3 (0.812) |
0.000552 0.000244 |
Purine nucleoside phosphorylase |
a
The microarray analysis reveals CD73 as the prominent candidate for further investigation.