Table IV.
Ecto-5′-nucleotidase activitya
| Cells | Jurkat | A4 | A4 | A4 | B-NT5.1 | B-NT5.1 | B-NT5.1 | B-NT5.1 | B-TM15.1 | B-TM15.1 |
|---|---|---|---|---|---|---|---|---|---|---|
| Treatment | Control | Control | AMPCP | PI-PLC | control | AMPCP | PI-PLC | control | AMPCP | PI-PLC |
| Ecto-5′-nucleotidase (nmol/106 cells/h) | Not detected | 2.75 ± 1.33 | 0.11 ± 0.20 | 0.43 ± 0.29 | 45.0 ± 9.35 | 0.97 ± 0.66 | 15.2 ± 6.05 | 154 ± 19.0 | 3.5 ± 2.24 | 131.2 ± 40 |
a
Activity was measured in parental Jurkat cells, multirésistant A4 cells, and Jurkat cell clones B-NT5.1 and B-TM15.1, stably transfected with GPI-anchored and transmembrane CD73, respectively (mean ± SD; n = 3). Some cells were preincubated for 30 min at 37°C with rPI-PLC (2.5 U/ml) from Bacillus cereus, or treated with AMPCP (1 mM).