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. 2017 Aug 30;95(5):1048–1055.e3. doi: 10.1016/j.neuron.2017.08.018

Figure 3.

Figure 3

Further Examples of Robotic Two-Photon Targeted Whole-Cell Recording In Vivo

(A and B) Current-clamp traces (A) during current injection (400 ms-long pulses from −100 to +100 pA in 50 pA steps) and (B) at rest for a patched neocortical interneuron in the V1 cortex of a GAD67-gfp (depth −113 μm from the brain surface; note compressed timescale relative to A).

(C) Maximum-intensity z-projection of a two-photon stack image of the patched neuron and the electrode, acquired after the recording.

(D and E) Current-clamp traces (D) during current injection (400 ms-long pulses from −100 to +25 pA in 25 pA steps) and (E) at rest for a patched neuron in the V1 cortex of a GAD67-gfp (depth −102 μm from the brain surface).

(F) Maximum-intensity z-projection of a two-photon stack image of the patched neocortical interneuron and the electrode, acquired after the recording.

(G and H) Current-clamp traces (G) during current injection (400 ms long pulses from −100 to +150 pA in 50 pA steps) and (H) at rest for a patched neuron in the V1 cortex of a GAD67-gfp (depth −142 μm from the brain surface).

(I) Maximum-intensity z-projection of a two-photon stack image of the patched neocortical interneuron and the electrode, acquired after the recording.