REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental Models: Organisms/Strains
Mouse: C57BL/6	Harlan UK and JAX	RRID: IMSR_JAX:000664
Mouse: GAD67-Gfp	Tamamaki et. al., 2003	GAD67-Gfp Mouse; MGI:3590301; RRID: IMSR_RBRC03666
Software and Algorithms
Labview software	National Instrument	Labview 2012; RRID: SCR_014325
MATLAB software	MathWorks	MATLAB 2013a; RRID: SCR_001622
Sciscan software (ver 11 Dec 2014)	Scientifica	N/A
Software and relative dll file for third parties software interface	Axon Instruments	Multiclamp commander
Robotic Integrated Targeted Autopatcher (RITA) software	This paper	https://github.com/schultzlab/rita/
Other
Two photon microscope	Scientifica	N/A
Galvanometric scanning mirrors	Cambridge Technology	6215H-671XX
Photo-multiplier tubes	Hamamatsu Photonics	R3896s
Dichroic beam splitter	Chroma Technology	T565LPXR
Optical filters	Chroma Technology	ET620/60M-2P
Optical filters	Chroma Technology	ET525/50M-2P
Mode-locked Ti-sapphire laser	Spectraphysics	Mai Tai HP
Water immersion objective, 0.8 N.A., 3.3 mmW.D.,	Olympus	LUMPLFLN-W 40 ×
Objective, plan achromat, 0.1 N.A., 18.5 mm W.D.	Olympus	RMS 4x
24 V / 65 W power supply	Maplin	N10NB
CCD Camera (based on a Sony CCD Progressive Scan Chip)	Scientifica	SciCam CCD Camera
Digital/analog acquisition board	National Instruments	DAQ PCI-6110
Digital/analog acquisition board	National Instruments	DAQ USB-6211
3axis micromanipulator	Scientifica	Multistar
Signal amplifier	Axon Instruments	Multiclamp 700B
Microcontroller (Arduino Due based on the Atmel SAM3X8E ARM Cortex-M3 CPU).	RS	Arduino Due
Custom assembled personal computer (Based on Microsoft Windows 7).	Scientifica	N/A
Pressure tap	Beefittings	FRLI 1/4, 2000 L/m
Flow control studs elbow	SMC Pneumatics	AS 2211F-01
Proportional solenoid valves	SMC Pneumatics	PVQ33-5G-16
Venturi vacuum ejector	SMC Pneumatics	ZH10DL-06-06-08
Flow control stud elbow	Beefittings	BSPT-PCSET 06-1/8
3 port solenoid valve	SMC Pneumatics	VQ110U-5M-Q
A Polytetrafluoroethylene (PTFE) tube (internal diameter 1.5 mm, wall thickness 0.3 mm)	VWE	DENE3401521
Bipolar NPN power transistors	ST Microelectronics	BD 237
NPN Darlington transistor	Digikey	TD - ZTX605
Diode	Transys Electronics	IN916
Pressure sensor	SMC Pneumatics	PSE 543-M3
Rectal probe and a feedback-controlled heating pad	FHC	40-90-8D
Dissecting microscope	Leica	S6E
Stereotaxic frame for small rodents	Harvard apparatus	724793
Dental drill	Osada Group	Success 40
Dumont Forceps – Dumostar size = 0.05 × 0.02 mm;	Fine Science Tools	11295-10
Ag/AgCl half-cell electrode 1.0 mm × 2.5 mm	World Precision Instruments	EP1
Manual pipette (0.5-10 μL)	Eppendorf	ES-10
Microloader tips (0.5 – 20 μL, 100 mm)	Eppendorf	Catalog No. 5242956003
Borosilicate capillaries with filament (76 mm, 1.5 mm outer diameter, 0.84 mm inner diameter)	World Precision Instrument	1B150F-3
Histoacrylamide	Braun Corporation	TS1050044FP
Dental acrylic	Kemdent	ACR811
Ophthalmic ointment	Allergan	Lacri-lube
Fentanyl (50 mg/ml)	Hameln pharmaceutical, UK	Sublimase
Midazolam (10 mg/ml)	Roche Products, UK	Hypnovel
Medetomidin (10 mg/ml)	Vetquinol, UK	Domitor
Alexa Fluor 594 hydrazide	Thermo Fisher and Molecular Probes	A10438