Fig. 2.

Deletion mutants of MGAT1 have no effect on enzyme activity. (A) Immunoblot analysis of total membrane fraction isolated from COS-7 cells transfected with the indicated deletion mutants (left). The enzymatic activity determined in membrane fraction by measuring the fluorescence signal (right). (B) Alignment of partial sequence of MGAT1 members. A highly conserved HPHG sequence is indicated by a blue box. (C) The enzymatic activity determined in membrane fraction by measuring the fluorescence signal. (D) Immunofluorescence microscopy of lipid droplets in COS-7 cells treated with oleate. These cells were labeled with the neutral lipid stain BODIPY 495/503 (green). One representative experiment of three is shown.