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. 2017 Sep 5;12(9):e0184026. doi: 10.1371/journal.pone.0184026

Fig 4. H. pylori uptake by AGS cells.

Fig 4

(A) Flow cytometry was used to assess internalization of DiO-labelled bacteria following infection of AGS cells with H. pylori (MOI, 10:1) for 15 h. (B) Infected cells were treated with gentamycin for 2 h to kill any remaining extracellular bacteria. Quantitative assessment of viable H. pylori in AGS cells was by serial dilutions of cells lysates. Results are ± SEM of three independent experiments. *, results are statistically different from those of cells infected with H. pylori wildtype strain 60190 (P<0.05), as determined by one-way ANOVA with Tukey’s test for multiple comparisons.