Skip to main content
. 2017 Sep 5;12(9):e0184026. doi: 10.1371/journal.pone.0184026

Fig 5. Labile iron pool in AGS cells infected with H. pylori CagA and VacA mutants.

Fig 5

AGS cells infected with H. pylori (MOI, 10:1) for 15 h were (A) fixed and stained with sulphide-silver to detect lysosomal iron or (B), treated with calcein with and without the addition of SIH to measure the cytosolic labile iron pool. (A) The mean pixel intensity (MPI) of each image was calculated using ImageJ software and evidence of lysosomal iron in infected cells (indicated by MPI) was expressed as a percentage of that observed in uninfected (Ctrl) cells. (B) Results reflect the increase in fluorescence (ΔF) in cells infected with the wildtype, and VacA and CagA mutant strains of H. pylori strain 60190 presented as a percentage of uninfected cells. (A) Results are ± SEM of five images taken from three independent experiments and (B), ± SEM of 3 independent repeats. *, **, ****, results are statistically different from those of untreated controls (P<0.05, <0.01 and <0.0001, respectively), as determined by one-way ANOVA with Tukey’s test for multiple comparisons.