Table 3.
Effect of media composition and photoperiod on asymbiotic germination of Chloraea crispa seeds 60, 120, and 180 d after sowing. Germination efficiency was estimated considering calli formation and germination of protocorms.a
| 60 d | 120 d | 180 d | ||||
| Treatmentb | Embryogenic calli | Germinated protocorms | Embryogenic calli | Germinated protocorms | Embryogenic calli | Germinated protocorms |
| 16 h light: 8 h dark | ||||||
| I-1 | 5.50 ± 0.60 b | 0.00 d | 5.50 ± 1.00 c | 13.00 ± 2.00 a | 3.00 ± 2.00 de | 24.00 ± 0.86 a |
| I-2 | 17.30 ± 1.52 a | 2.80 ± 1.60 c | 10.80 ± 3.80 a | 7.00 ± 0.00 b | 11.00 ± 2.00 a | 7.50 ± 1.00 cd |
| I-3 | 4.00 ± 1.80 b | 0.00 d | 5.50 ± 1.00 c | 0.00 d | 6.70 ± 1.50 bc | 1.75 ± 1.50 e |
| I-4 | 4.80 ± 0.60 b | 0.00 d | 6.00 ± 0.00 c | 11.00 ± 3.70 ab | 7.50 ± 1.00 b | 13.30 ± 2.50 b |
| I-5 | 0.00 b | 0.00 d | 0.00 d | 0.00 d | 0.00 e | 0.00 f |
| Continuous darkness | ||||||
| I-1 | 6.80 ± 3.50 b | 16.50 ± 1.00 a | 3.70 ± 2.90 c | 17.00 ± 3.50 a | 3.70 ± 2.90 c | 21.00 ± 3.00 a |
| I-2 | 0.00 c | 0.00 d | 8.00 ± 0.00 ab | 6.50 ± 1.00 bc | 10.30 ± 2.20 ab | 8.00 ± 0.00 c |
| I-3 | 6.70 ± 3.20 b | 5.00 ± 1.00 b | 7.50 ± 0.58 ab | 5.70 ± 0.50 c | 9.20 ± 1.70 ab | 6.50 ± 1.00 d |
| I-4 | 3.70 ± 2.90 b | 4.60 ± 2.90 bc | 3.70 ± 2.80 c | 14.00 ± 2.60 a | 3.70 ± 0.60 d | 16.00 ± 0.80 b |
| I-5 | 4.00 ± 0.80 b | 4.00 ± 3.30 bc | 4.70 ± 0.50 c | 4.00 ± 1.10 c | 5.00 ± 0.00 c | 4.00 ± 1.00 d |
a
Different letters in the same column signify significant differences between treatments (Tukey’s honest significant difference test, P ≤ 0.05).
b
I-1 = BM-2 (Van Waes basal medium [Van Waes and Debergh, 1986]); I-2 = 50% MS; I-3 = BM-2 + 1.0 mg·L−1 CaNO3 + 0.5 g·L−1 (NH4)2SO4; I-4 = BM-2 + 0.2 mg·L−1 BAP; I-5 = BM-2 + 0.2 mg·L−1 IBA.