Table 5.

Effect of plant growth regulators and photoperiod on calli induction and asymbiotic germination of Chloraea crispa seeds 60, 120, and 180 d after sowing. Germination efficiency was estimated considering calli formation and germination of protocorms.^a

	60 d		120 d		180 d
Treatmentb	Embryogenic calli	Germinated protocorms	Embryogenic calli	Germinated protocorms	Embryogenic calli	Germinated protocorms
16 h light: 8 h dark
II-1	5.00 ± 0.82 ab3	0.00 c	2.50 ± 0.58 d	14.00 ± 0.82 b	3.50 ± 0.58 c	26.00 ± 1.63 b
II-2	1.50 ± 1.29 c	0.00 c	5.50 ± 1.91 bc	1.00 ± 0.00 f	8.00 ± 1.41 b	3.00 ± 1.63 f
II-3	0.00 d	0.00 c	3.00 ± 0.82 cd	3.00 ± 0.82 e	2.50 ± 0.58 cd	3.00 ± 0.82 f
II-4	0.00 d	0.00 c	2.50 ± 0.58 d	2.00 ± 0.82 e	1.50 ± 1.00 d	4.50 ± 1.00 f
Continuous darkness
II-1	4.50 ± 1.92 ab	4.50 ± 1.91 b	15.50 ± 3.51 a	15.50 ± 1.00 b	19.00 ± 1.41 a	21.00 ± 1.63 c
II-2	3.50 ± 0.58 b	8.50 ± 1.00 a	1.50 ± 0.58 d	27.50 ± 2.08 a	1.50 ± 0.58 d	30.50 ± 1.73 a
II-3	5.00 ± 2.31 ab	6.50 ± 0.58 ab	7.00 ± 0.00 b	6.50 ± 0.58 d	10.50 ± 1.00 b	6.00 ± 0.00 e
II-4	7.00 ± 1.41 a	4.00 ± 0.00 b	3.50 ± 1.30 cd	10.00 ± 0.00 c	3.50 ± 1.30 c	15.00 ± 0.58 d

^aDifferent letters in the same column signify significant differences between treatments (Tukey’s honest significant difference test, P ≤ 0.05).

^bII-1 = BM-2 (Van Waes basal medium [Van Waes and Debergh, 1986]); II-2 = BM-2 + 0.1 mg·L⁻¹ BAP; II-3 = BM-2 + 0.1 mg·L⁻¹ IBA; II-4 = BM-2 + 0.2 mg·L⁻¹ BAP + 0.1 mg·L⁻¹ IBA.