Appendix 2.
Twelve-base Golay error-correcting barcode sequences that passed in silico testing for secondary structure formation. Individual barcode sequences from this list are substituted for XXXXXXXXXXXX in the “Golay barcode” region (Table 2) to generate indexed reverse primer constructs for PCR.
| Barcode name | Barcode nucleotide sequence | Barcode name | Barcode nucleotide sequence | Barcode name | Barcode nucleotide sequence |
| AML2_il001 | TCCCTTGTCTCC | AML2_il027 | AGTTACGAGCTA | AML2_il053 | CGGTCAATTGAC |
| AML2_il002 | GCTGTACGGATT | AML2_il028 | GCATATGCACTG | AML2_il054 | GTGGAGTCTCAT |
| AML2_il003 | ATCACCAGGTGT | AML2_il029 | CAACTCCCGTGA | AML2_il055 | GCTCGAAGATTC |
| AML2_il004 | TGGTCAACGATA | AML2_il030 | TTGCGTTAGCAG | AML2_il056 | AGGCTTACGTGT |
| AML2_il005 | ATCGCACAGTAA | AML2_il031 | TACGAGCCCTAA | AML2_il057 | TCTCTACCACTC |
| AML2_il006 | AGCGGAGGTTAG | AML2_il032 | CACTACGCTAGA | AML2_il058 | ACTTCCAACTTC |
| AML2_il007 | ATCCTTTGGTTC | AML2_il033 | TGCAGTCCTCGA | AML2_il059 | CTCACCTAGGAA |
| AML2_il008 | TACAGCGCATAC | AML2_il034 | ACCATAGCTCCG | AML2_il060 | GTGTTGTCGTGC |
| AML2_il009 | ACCGGTATGTAC | AML2_il035 | TCGACATCTCTT | AML2_il061 | CCACAGATCGAT |
| AML2_il010 | AATTGTGTCGGA | AML2_il036 | GAACACTTTGGA | AML2_il062 | TATCGACACAAG |
| AML2_il011 | TGCATACACTGG | AML2_il037 | GAGCCATCTGTA | AML2_il063 | GATTCCGGCTCA |
| AML2_il012 | AGTCGAACGAGG | AML2_il038 | TAATACGGATCG | AML2_il064 | TAGGCATGCTTG |
| AML2_il013 | ACCAGTGACTCA | AML2_il039 | TCGGAATTAGAC | AML2_il065 | AACTAGTTCAGG |
| AML2_il014 | GAATACCAAGTC | AML2_il040 | TGTGAATTCGGA | AML2_il066 | GTACGATATGAC |
| AML2_il015 | GTAGATCGTGTA | AML2_il041 | TACTACGTGGCC | AML2_il067 | TAGTATGCGCAA |
| AML2_il016 | CCAATACGCCTG | AML2_il042 | GGCCAGTTCCTA | AML2_il068 | ATGGCTGTCAGT |
| AML2_il017 | GATCTGCGATCC | AML2_il043 | GATGTTCGCTAG | AML2_il069 | GCGTTCTAGCTG |
| AML2_il018 | CAGCTCATCAGC | AML2_il044 | CTATCTCCTGTC | AML2_il070 | GTTGTTCTGGGA |
| AML2_il019 | CAAACAACAGCT | AML2_il045 | ACTCACAGGAAT | AML2_il071 | ATGTCACCGCTG |
| AML2_il020 | GCAACACCATCC | AML2_il046 | ATGATGAGCCTC | AML2_il072 | AGCAGAACATCT |
| AML2_il021 | CGAGCAATCCTA | AML2_il047 | GTCGACAGAGGA | AML2_il073 | TGGAGTAGGTGG |
| AML2_il022 | AGTCGTGCACAT | AML2_il048 | TGTCGCAAATAG | AML2_il074 | TTGGCTCTATTC |
| AML2_il023 | GTATCTGCGCGT | AML2_il049 | CATCCCTCTACT | AML2_il075 | GATCCCACGTAC |
| AML2_il024 | CGAGGGAAAGTC | AML2_il050 | TATACCGCTGCG | AML2_il076 | TACCGCTTCTTC |
| AML2_il025 | CAAATTCGGGAT | AML2_il051 | AGTTGAGGCATT | AML2_il077 | TGTGCGATAACA |
| AML2_il026 | AGATTGACCAAC | AML2_il052 | ACAATAGACACC | AML2_il078 | GATTATCGACGA |
| AML2_il079 | GCCTAGCCCAAT |