Figure 5.

Biological functions of Gcn5. (A) Banding pattern of the WT, gcn5Δ and GCN5OX strains. Closed bar represents the growth phase in dark, and open bar for growth under light. Five dark-light (12 h-12 h) cycles were given following growth in constant dark for 3 d. The PA medium supporting mycelial growth was kept at 60°C for 3 to 5 h, for fast dehydration to achieve better contrast of the banding, before photography. (B) Bar chart depicting quantitatively assessed conidiation in the indicated strains, grown in dark or light conditions. Mean values ( ± SE) presented as percentage points were derived from 3 independent experiments (n = 15 colonies for each sample). (C) TFB5 transcripts in WT, gcn5Δ and GCN5OX cultures exposed to light for 0, 2, 4 and 6 h. Total RNA (2 μg) serves as a loading control. Primers used for RT-PCR are listed in Table S1. (D) Bar chart depicting quantitatively assessed conidiation in the indicated strains. Mean values ( ± SE) presented as percentage points were derived from 3 independent experiments (n = 15 colonies for each sample). (E) Barley leaf explants (intact or wounded) were inoculated with conidia from the indicated strains and disease symptoms assessed after 7 d. (F) Sensitivity toward oxidative stress was tested in the WT, gcn5Δ and GCN5OX strains, cultured on PA solid medium supplemented with H₂O₂ at concentration of 0 (control), 1, 2.5, 5, and 10 mM.